Biology Department Instrumentation

Autoclave_VLW417

Directions for using the autoclave in Wharton Hall 417

Follow the steps below to use the autoclave:

  • Book the autoclave based on the cycle number you'd like to use (see below)
  • If the screen is dark just touch it and it will light up!
  • Check the pressure gauge is down to zero
  • Load the autoclave. Be sure to put anything that will boil over in a container. Be sure to put autoclavable trash bags in a large container
  • Gently close the door! Hold on to it while you close it!

AutoclaveMenu_VLW417
  • Choose your cycle number:
    1. Liquids (used for anything that has water in it and has a 15min exposure time)
    2. Dry (15 min exposure time)
    3. Trash (45 min exposure and should be used for the big bags that have media that will melt and become liquid)
    4. Tips (15 min exposure time with an hour drying time)

  • Press start and the time remaining will appear in a circle on the display
  • Come back after the cycle, make sure the pressure if down to zero, and remove the contents. Autoclaved trash should be discarded into the provided trash can.
  • If the media boils over, turn the autoclave off (green button on the right) and use water to clean the chamber. There are three drains in the bottom of the autoclave. Make sure they are not blocked.
If you have any questions contact , phone: 337-344-1419.